THE FUCHSIN PUFFER STAINING OF ACID-FAST BACTERIUM IN TISSUE

Inefficiency of 0.3% carbol fuchsin in ziehl-neelsen staining for detecting acid-fast bacilli.

We compared the sensitivity and specificity of a modified Ziehl-Neelsen (modified-ZN) staining method for acid-fast bacilli (AFB) with that of the standard Ziehl-Neelsen (standard-ZN) staining method, using culture results with Mycobacterium tuberculosis as the "gold standard." The sensitivity (72%; 101 of 140) of the modified-ZN staining method, which uses 0.3% carbol fuchsin, was significantl...

Variability of haematoxylin - basic fuchsin - picric acid (HBFP) staining of infarcted myocardium due to tissue storage.

Early myocardial infarction is usually detected (3) The effect of storage and drying on the timgrossly by utilising the nitro-blue tetrazolium (NBT) ing of the differentiation step was also technique.1 The normal areas stain dark blue and studied. infarcted areas which lack the dehydrogenase enzyme remain unstained. In 1971, Lie et aI2 described a non-enzymatic histochemical method, the haemato...

Phenol ammonium sulphate basic fuchsin staining of sputum in pot for the detection of acid-fast bacilli.

BACKGROUND & OBJECTIVES Improper practices of making direct smears of sputum for detection of acid-fast bacilli (AFB) and of disposing sputum cups are hazardous. The present study was undertaken with the objective to stain sputum samples in their containers by 'phenol (10%) ammonium sulphate (4%) basic fuchsin (2%) solution' and to decolourize and counterstain their smears for detection of AFB-...

Comparison of two different strengths of carbol fuchsin in Ziehl-Neelsen staining for detecting acid-fast bacilli.

An improved periodic acid fuchsin sulfite staining method for evaluation of glycoproteins.

01W AND GRONWALL (1) [N 1952 first applied the histochemical method of Hotchkiss for staining carbohydrates. In principle the polysaccharides are oxidized by periodic acid to give polyaldehydes, which yield violet-red compounds with Schiff ‘s reagent, fuchsin sulfite. K#{246}iw’s procedure, although giving excellent results when the glycoprotein was separated electrophoretically on filter paper...